7MLX

SARS-CoV-2 programmed -1 frameshifting element three stem H-type pseudoknot

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-11-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979180
Spacegroup name	C 2 2 21
Unit cell lengths	76.833, 143.240, 133.641
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.620 - 2.090
R-factor	0.2008
Rwork	0.199
R-free	0.23090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Antibody Fragment model
RMSD bond length	0.007
RMSD bond angle	0.943
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.620	2.165
High resolution limit [Å]	2.090	2.090
Number of reflections	44027	4321
<I/σ(I)>	15.98
Completeness [%]	99.7	99.93
Redundancy	6.8
CC(1/2)	0.999	0.803

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	2 % v/v tacsimate pH 4, 0.1 M sodium acetate trihydrate pH 4.6, 16 % w/v polyethylene glycol 3350