7MKY

SARS-CoV-2 frameshifting pseudoknot RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-10
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.8266
Spacegroup name	C 1 2 1
Unit cell lengths	113.371, 39.206, 38.803
Unit cell angles	90.00, 97.98, 90.00

Refinement procedure

Resolution	38.430 - 1.310
R-factor	0.1551
Rwork	0.154
R-free	0.18770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7lyj
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.430	38.430	1.220
High resolution limit [Å]	1.200	6.570	1.200
Rmerge	0.054	0.041	2.251
Rmeas	0.063	0.047	2.733
Rpim	0.032	0.023	1.519
Total number of observations	175993	1121	7340
Number of reflections	51812	311	2513
<I/σ(I)>	8.6	27.2	0.4
Completeness [%]	98.2	91.8	96.6
Redundancy	3.4	3.6	2.9
CC(1/2)	0.998	0.997	0.125

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	34-40% (v/v) MPD, 40 mM sodium cacodylate, pH 5.5, 20 mM magnesium chloride, 1 mM cobalt hexammine