7MKS
Crystal structure of the GH12 domain from Acidothermus cellulolyticus GuxA bound to cellobiose
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER X8 PROTEUM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-28 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 66.235, 66.235, 127.176 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.100 - 1.850 |
| Rwork | 0.133 |
| R-free | 0.16490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b7m |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.776 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.100 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.038 | 0.292 |
| Number of reflections | 28178 | 2727 |
| <I/σ(I)> | 17.2 | 2.8 |
| Completeness [%] | 99.7 | 95.5 |
| Redundancy | 1.9 | 1.7 |
| CC(1/2) | 0.998 | 0.755 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 10% PEG8000, 0.2 M zinc chloride, 0.1 M sodium cacodylate |
