7MKR
Crystal structure of the GH12 domain from Acidothermus cellulolyticus GuxA
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER X8 PROTEUM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-21 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 66.182, 66.182, 127.893 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.980 - 1.500 |
| Rwork | 0.129 |
| R-free | 0.17110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b7m |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.049 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.980 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.052 | 0.329 |
| Number of reflections | 52722 | 2501 |
| <I/σ(I)> | 10.3 | 2.2 |
| Completeness [%] | 99.7 | 97.4 |
| Redundancy | 1.9 | 1.8 |
| CC(1/2) | 0.997 | 0.654 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 10% PEG8000, 0.2 M zinc chloride, 0.1 M sodium cacodylate |
