7MK7
Augmentor domain of augmentor-beta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979200 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.569, 56.775, 94.408 |
| Unit cell angles | 75.60, 77.35, 61.78 |
Refinement procedure
| Resolution | 46.869 - 2.428 |
| R-factor | 0.21730694854 |
| Rwork | 0.214 |
| R-free | 0.27279 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1anf |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.747 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.570 |
| High resolution limit [Å] | 2.428 | 2.428 |
| Rmeas | 0.131 | |
| Number of reflections | 33843 | 5220 |
| <I/σ(I)> | 10.12 | 2.07 |
| Completeness [%] | 94.8 | 90.1 |
| Redundancy | 6.7 | 6.5 |
| CC(1/2) | 0.995 | 0.806 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 293 | 0.1 M sodium citrate pH 4.9, 16% PEG 4000 |
