7MK6
KcsA open gate E71V mutant with sodium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 98 |
| Detector technology | PIXEL |
| Collection date | 2021-03-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | I 4 |
| Unit cell lengths | 140.139, 140.139, 69.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.100 |
| R-factor | 0.2107 |
| Rwork | 0.208 |
| R-free | 0.25260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vk6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.839 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.090 | 3.310 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 12391 | 2218 |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.4 | |
| CC(1/2) | 0.993 | 0.165 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50 mM sodium/ammonium acetate pH 5.5, 50 mM Magnesium acetate, 25% PEG 400 |
