7MJX
MiaB in the complex with 5'-deoxyadenosine, methionine and RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.690, 97.120, 80.606 |
| Unit cell angles | 90.00, 108.10, 90.00 |
Refinement procedure
| Resolution | 41.020 - 1.500 |
| R-factor | 0.1488 |
| Rwork | 0.148 |
| R-free | 0.17010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qgq |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.042 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 3.700 | 1.500 |
| Rmerge | 0.055 | 0.040 | 0.244 |
| Rmeas | 0.071 | 0.051 | 0.325 |
| Rpim | 0.044 | 0.031 | 0.212 |
| Total number of observations | 289708 | ||
| Number of reflections | 143421 | 10258 | 8886 |
| <I/σ(I)> | 10.1 | ||
| Completeness [%] | 89.3 | 94.1 | 83.4 |
| Redundancy | 2 | 2.3 | 1.7 |
| CC(1/2) | 0.994 | 0.872 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100 mM sodium cacodylate, pH 5.5, 13% PEG 4000 and 3% (+/-)-2-methyl-2,4-pentanediol |
