7MJX

MiaB in the complex with 5'-deoxyadenosine, methionine and RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0033
Spacegroup name	P 1 21 1
Unit cell lengths	68.690, 97.120, 80.606
Unit cell angles	90.00, 108.10, 90.00

Refinement procedure

Resolution	41.020 - 1.500
R-factor	0.1488
Rwork	0.148
R-free	0.17010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qgq
RMSD bond length	0.018
RMSD bond angle	2.042
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.530
High resolution limit [Å]	1.500	3.700	1.500
Rmerge	0.055	0.040	0.244
Rmeas	0.071	0.051	0.325
Rpim	0.044	0.031	0.212
Total number of observations	289708
Number of reflections	143421	10258	8886
<I/σ(I)>	10.1
Completeness [%]	89.3	94.1	83.4
Redundancy	2	2.3	1.7
CC(1/2)		0.994	0.872

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	100 mM sodium cacodylate, pH 5.5, 13% PEG 4000 and 3% (+/-)-2-methyl-2,4-pentanediol