7MJV

MiaB in the complex with s-adenosylmethionine and RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-24
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.0033
Spacegroup name	P 21 21 21
Unit cell lengths	54.155, 81.442, 109.751
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.510 - 2.240
R-factor	0.2456
Rwork	0.243
R-free	0.27260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qgq
RMSD bond length	0.008
RMSD bond angle	1.887
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.330
High resolution limit [Å]	2.240	4.850	2.250
Rmerge	0.153	0.087	0.543
Rmeas	0.164	0.093	0.600
Rpim	0.058	0.031	0.250
Number of reflections	22398	2561	1609
<I/σ(I)>	7.6
Completeness [%]	93.7	99.6	68.4
Redundancy	7.3	8.4	4
CC(1/2)		0.996	0.905

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	100 mM Na-cacodylate, pH 5.5, 13% PEG 4000 and 3% (+/-)-2-methyl-2,4-pentanediol