7MJU

Crystal structure of human AF10 PZP bound to histone H3 tail

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2018-08-24
Detector	RDI CMOS_8M
Wavelength(s)	1.27819
Spacegroup name	P 1 21 1
Unit cell lengths	42.920, 54.330, 47.780
Unit cell angles	90.00, 106.82, 90.00

Refinement procedure

Resolution	41.084 - 2.100
R-factor	0.1668
Rwork	0.165
R-free	0.20590
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.828
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.090	2.160
High resolution limit [Å]	2.100	2.100
Number of reflections	12143	845
<I/σ(I)>	15.9
Completeness [%]	97.9
Redundancy	13.2
CC(1/2)	0.998	0.637

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.09 M Tris, 22.5% PEG 3350, and 10 mM spermine tetrahydrochloride