7MIE

Crystal structure of the Borreliella burgdorferi PlzA protein in complex with c-di-GMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	63.699, 36.861, 64.905
Unit cell angles	90.00, 113.90, 90.00

Refinement procedure

Resolution	32.260 - 1.600
R-factor	0.1921
Rwork	0.191
R-free	0.21760
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.716
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.260	50.000	1.630
High resolution limit [Å]	1.600	4.340	1.600
Rmerge	0.086	0.062	0.573
Rmeas	0.095	0.068	0.635
Rpim	0.040	0.028	0.268
Number of reflections	35870	1905	1619
<I/σ(I)>	8.3
Completeness [%]	97.7	98.4	89.3
Redundancy	5.7	5.9	4.5
CC(1/2)		0.995	0.871

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.1 M succinic acid pH 7.0, 15% (w/v) PEG 3350