7MIC

Maize rayado fino virus protease in complex with Ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2019-07-30
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.541870
Spacegroup name	I 4
Unit cell lengths	75.720, 75.720, 79.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.160 - 2.090
R-factor	0.2107
Rwork	0.205
R-free	0.26280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7mia IUBQ
RMSD bond length	0.002
RMSD bond angle	0.533
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.160	2.170
High resolution limit [Å]	2.090	2.090
Number of reflections	13343	1341
<I/σ(I)>	26.62
Completeness [%]	96.6
Redundancy	2
CC(1/2)	0.910	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.8	277	100 mM phosphate citrate buffer (pH 3.8), 200 mM lithium sulfate and 25 % PEG 1000