7MHU
Sialidase24 apo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 41 |
| Unit cell lengths | 141.109, 141.109, 54.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.620 - 2.000 |
| R-factor | 0.196 |
| Rwork | 0.189 |
| R-free | 0.20540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold T1089TS427_2 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.734 |
| Data reduction software | MOSFLM (7.3.0) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHENIX (1.19.1_4122) |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.620 | 2.040 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.272 | |
| Number of reflections | 73015 | 4481 |
| <I/σ(I)> | 6.4 | |
| Completeness [%] | 100.0 | |
| Redundancy | 12.3 | |
| CC(1/2) | 0.994 | 0.100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 100 mM Bis-Tris, 20% polyethylene glycol monomethyl ether 5000 |
