7MHT
CYTOSINE-SPECIFIC METHYLTRANSFERASE HHAI/DNA COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 99.860, 99.860, 325.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.000 - 2.870 |
| R-factor | 0.156 |
| Rwork | 0.156 |
| Structure solution method | OTHER |
| Starting model (for MR) | 5mht |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 23.000 |
| High resolution limit [Å] | 2.870 |
| Rmerge | 0.066 |
| Total number of observations | 55718 * |
| Number of reflections | 13031 |
| <I/σ(I)> | 13.3 |
| Completeness [%] | 90.0 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.25 * | 16 * | 1.4-1.8 M AMMONIUM SULFATE 50 MM CITRATE PH 5.6 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | (NH4)2SO4 | ||
| 2 | 1 | 1 | CITRATE | ||
| 3 | 1 | 2 | (NH4)2SO4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | ammonium sulfate | 1.3-1.7 (M) | ||
| 2 | 1 | sodium citrate | 50 (mM) | ||
| 3 | 1 | protein | 10 (mg/ml) | ||
| 4 | 1 | 2 | 50 (mM) | ||
| 5 | 1 | 2 | Tris-HCl | 20 (mM) | |
| 6 | 1 | 2 | 100 (mM) | ||
| 7 | 1 | 2 | Na2EDTA | 1 (mM) | |
| 8 | 1 | 2 | 2-mercaptoethanol | 0.1 (%) | |
| 9 | 1 | 2 | AdoHcy |
