7MH6

Structure of EmrE-D3 mutant in complex with monobody L10 in low pH (protonated state)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-10-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	.987
Spacegroup name	C 1 2 1
Unit cell lengths	140.641, 49.850, 109.830
Unit cell angles	90.00, 93.75, 90.00

Refinement procedure

Resolution	35.080 - 2.850
R-factor	0.3096
Rwork	0.309
R-free	0.33000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wk8
Data reduction software	MOSFLM
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.170	3.160
High resolution limit [Å]	2.850	2.850
Number of reflections	11183	560
<I/σ(I)>	9.5
Completeness [%]	87.0	62.6
Redundancy	6.4	7.1
CC(1/2)	0.994	0.366

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM sodium chloride, 100 mM sodium cacodylate, pH 5.5, 34% PEG600