7MGT
Ftp from Treponema pallidum bound to an ADP-like inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 116.483, 47.106, 57.472 |
Unit cell angles | 90.00, 102.30, 90.00 |
Refinement procedure
Resolution | 35.840 - 1.540 |
R-factor | 0.1525 |
Rwork | 0.150 |
R-free | 0.19770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ifu |
RMSD bond length | 0.006 |
RMSD bond angle | 0.961 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.844 | 1.570 |
High resolution limit [Å] | 1.540 | 1.540 |
Number of reflections | 43888 | 2200 |
<I/σ(I)> | 40.8 | |
Completeness [%] | 97.0 | |
Redundancy | 6.8 | |
CC(1/2) | 0.994 | 0.931 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M magnesium formate dihydrate 15% (w/v) PEG 3350 |