7MG0

Crystal structure of EV-D68 2A protease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2020-11-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 4
Unit cell lengths	118.157, 118.157, 81.735
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.880 - 3.500
R-factor	0.2432
Rwork	0.238
R-free	0.28510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7lw2
RMSD bond length	0.003
RMSD bond angle	0.707
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (dev_4189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.880	3.660
High resolution limit [Å]	3.200	3.200
Number of reflections	14387	931
<I/σ(I)>	25.4
Completeness [%]	99.9
Redundancy	3.9
CC(1/2)	0.988	0.477

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.05 M Sodium Cacodylate pH 6.0, 0.005 M Magnesium Chloride, 0.002 M Calcium Chloride, 15% v/v 2-Propanol, 0.001 M Spermine