7MFZ
The Crystal Structure of Domain-Swapped Trimer Q108K:K40D:T53A:R58L:Q38F:Q4F Mutant of HCRBPII Bound with LizFluor3 Chromophore Showing Excited State Intermolecular Proton Transfer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-26 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 36.932, 102.410, 108.478 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.960 - 2.490 |
R-factor | 0.2136 |
Rwork | 0.206 |
R-free | 0.28360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rct |
RMSD bond length | 0.008 |
RMSD bond angle | 1.189 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.960 | 2.579 |
High resolution limit [Å] | 2.490 | 2.490 |
Rmeas | 0.149 | 1.537 |
Number of reflections | 15016 | 1406 |
<I/σ(I)> | 18.88 | 1.75 |
Completeness [%] | 99.5 | 96.04 |
Redundancy | 8.5 | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 4000, sodium acetate , ammonium acetate |