7MFX
The Crystal Structure of Q108K:K40H:T53A:R58L:Q38F:Q4F Mutant of HCRBPII Bound with FR1 Chromophore Showing Excited State Intermolecular Proton Transfer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-13 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97624 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.687, 54.515, 63.931 |
| Unit cell angles | 90.00, 86.63, 77.69 |
Refinement procedure
| Resolution | 32.040 - 1.590 |
| R-factor | 0.1992 |
| Rwork | 0.198 |
| R-free | 0.23170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rct |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.874 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.040 | 1.651 |
| High resolution limit [Å] | 1.594 | 1.594 |
| Rmerge | 0.046 | 0.530 |
| Number of reflections | 55438 | 5085 |
| <I/σ(I)> | 22.5 | 2.12 |
| Completeness [%] | 86.8 | |
| Redundancy | 3.8 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG4000, sodium acetate, ammonium acetate |
