7MFW
Drosophila melanogaster Canoe PDZ domain in complex with Echinoid C-terminal region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 6 |
| Unit cell lengths | 114.563, 114.563, 31.637 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.500 - 2.104 |
| R-factor | 0.1956 |
| Rwork | 0.192 |
| R-free | 0.22560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3axa (Chain A res 1-91) |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.724 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.8) |
| Refinement software | PHENIX (1.8) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
| Rmerge | 0.071 | 0.038 | 0.474 |
| Rmeas | 0.074 | 0.040 | 0.497 |
| Rpim | 0.022 | 0.012 | 0.147 |
| Total number of observations | 154188 | ||
| Number of reflections | 13954 | 1452 | 1360 |
| <I/σ(I)> | 10.4 | ||
| Completeness [%] | 98.6 | 97.5 | 99.3 |
| Redundancy | 11 | 10.7 | 11.3 |
| CC(1/2) | 0.999 | 0.966 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 18% PEG 3350, 300 mM sodium acetate |
