Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 68.920, 68.920, 107.170 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.870 - 1.900 |
| R-factor | 0.2379 |
| Rwork | 0.237 |
| R-free | 0.25060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7KGL |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.951 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.074 | 1.800 |
| Rmeas | 0.077 | |
| Rpim | 0.022 | |
| Number of reflections | 12360 | 1025 |
| <I/σ(I)> | 15.19 | 0.69 |
| Completeness [%] | 94.5 | 85 |
| Redundancy | 12.4 | 12.6 |
| CC(1/2) | 0.999 | 0.526 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% PEG 4000, 0.1M MES pH 6.0, 0.2M LiSO4 |
