7MFN

N-Acetylmannosamine-6-phosphate 2-epimerase E180A from Staphylococcus aureus (strain MRSA USA300)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95366
Spacegroup name	P 21 21 2
Unit cell lengths	76.999, 174.446, 44.523
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.004 - 1.550
Rwork	0.150
R-free	0.17790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vva
RMSD bond length	0.014
RMSD bond angle	1.905
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.400	1.570
High resolution limit [Å]	1.550	1.550
Number of reflections	87948	8254
<I/σ(I)>	13.5
Completeness [%]	99.5
Redundancy	13.1
CC(1/2)	0.999	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	1 M trisodium citrate, 0.1 M sodium cacodylate, pH 6.5