7MFN
N-Acetylmannosamine-6-phosphate 2-epimerase E180A from Staphylococcus aureus (strain MRSA USA300)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95366 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 76.999, 174.446, 44.523 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.004 - 1.550 |
| Rwork | 0.150 |
| R-free | 0.17790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6vva |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.905 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.400 | 1.570 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 87948 | 8254 |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 99.5 | |
| Redundancy | 13.1 | |
| CC(1/2) | 0.999 | 0.842 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 1 M trisodium citrate, 0.1 M sodium cacodylate, pH 6.5 |
