7MF9

Crystal structure of antibody 10E8v4-P100fA Fab in space group C2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	93
Detector technology	PIXEL
Collection date	2019-08-09
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	160.967, 141.300, 137.413
Unit cell angles	90.00, 109.17, 90.00

Refinement procedure

Resolution	40.210 - 3.700
R-factor	0.2415
Rwork	0.240
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5iq9
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.760
High resolution limit [Å]	3.690	10.020	3.700
Rmerge	0.181	0.057	0.541
Rmeas	0.228	0.071	0.704
Rpim	0.136	0.042	0.446
Number of reflections	24124	1284	958
<I/σ(I)>	3.9
Completeness [%]	77.2	79.5	63.2
Redundancy	2.3	2.4	1.7
CC(1/2)		0.993	0.649

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	12% PEG 8000, 0.1M Tris-HCl, 8.5