7MF8

Crystal structure of antibody 10E8v4-P100fA Fab in space group P6422

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	93
Detector technology	PIXEL
Collection date	2019-08-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 64 2 2
Unit cell lengths	95.690, 95.690, 256.671
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.240 - 2.200
R-factor	0.2087
Rwork	0.207
R-free	0.23760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5iq9
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.200	5.970	2.200
Rmerge	0.085	0.057	0.911
Rmeas	0.090	0.061	0.971
Rpim	0.030	0.021	0.330
Total number of observations	322223
Number of reflections	36152	1973	1779
<I/σ(I)>	8
Completeness [%]	98.9	94.5	99.8
Redundancy	8.9	7.4	8.4
CC(1/2)		0.997	0.887

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	3.6M Sodium formate, 0.1M Sodium acetate 4.6