7MF7
Crystal structure of antibody 10E8v4-P100gA Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2019-07-12 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 220.216, 43.555, 226.462 |
| Unit cell angles | 90.00, 115.31, 90.00 |
Refinement procedure
| Resolution | 36.470 - 2.000 |
| R-factor | 0.2031 |
| Rwork | 0.203 |
| R-free | 0.23130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5iq9 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.040 |
| High resolution limit [Å] | 2.000 | 5.450 | 2.010 |
| Rmerge | 0.092 | 0.049 | 0.861 |
| Rmeas | 0.104 | 0.056 | 0.997 |
| Rpim | 0.046 | 0.027 | 0.492 |
| Number of reflections | 127970 | 6575 | 5820 |
| <I/σ(I)> | 7.6 | ||
| Completeness [%] | 97.3 | 94.8 | 89.4 |
| Redundancy | 5.1 | 4.2 | 3.7 |
| CC(1/2) | 0.996 | 0.486 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% PEG 8000, 1M Tris-HCl 8.5 |
