7MC6
Crystal structure of the SARS-CoV-2 ExoN-nsp10 complex containing Mg2+ ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.670, 70.320, 108.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.960 - 2.100 |
| R-factor | 0.1982 |
| Rwork | 0.197 |
| R-free | 0.21870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5c8t |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.404 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.850 | 47.850 | 1.710 |
| High resolution limit [Å] | 1.680 | 9.050 | 1.680 |
| Rmerge | 0.060 | 0.029 | 1.237 |
| Rmeas | 0.070 | 0.034 | 1.436 |
| Rpim | 0.036 | 0.018 | 0.724 |
| Total number of observations | 110232 | 711 | 5902 |
| Number of reflections | 29950 | 206 | 1550 |
| <I/σ(I)> | 11.2 | 38 | 1 |
| Completeness [%] | 97.8 | 96.9 | 96.4 |
| Redundancy | 3.7 | 3.5 | 3.8 |
| CC(1/2) | 0.998 | 0.998 | 0.509 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | MgCl2 , Tris-HCl pH 8.5, PEG 4000 |
