7MBW

Crystal structure of TnsC(1-503)A225V

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	CCD
Collection date	2019-08-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 21 21 21
Unit cell lengths	83.780, 95.770, 313.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.500 - 3.200
R-factor	0.2186
Rwork	0.216
R-free	0.25850
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.667
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.770	3.320
High resolution limit [Å]	3.200	3.200
Number of reflections	42690	4414
<I/σ(I)>	9.7	2.3
Completeness [%]	100.0	100
Redundancy	14.5	14.7
CC(1/2)	0.990	0.390

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		295	23-28% PEG 5000 MME, 0.2M Ammonium sulfate, 3% glycerol, 0.1M MES