7MBJ
Crystal structure of cGMP dependent protein kinase I alpha (PKG I alpha)CNB-A domain with R177Q mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.198, 41.855, 48.312 |
| Unit cell angles | 94.74, 113.66, 114.03 |
Refinement procedure
| Resolution | 36.540 - 1.260 |
| R-factor | 0.1785 |
| Rwork | 0.177 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3shr |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.177 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.310 |
| High resolution limit [Å] | 1.260 | 1.260 |
| Rmerge | 0.055 | 0.175 |
| Number of reflections | 62350 | 5426 |
| <I/σ(I)> | 25.04 | 6.56 |
| Completeness [%] | 93.6 | 80.9 |
| Redundancy | 3.7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 277 | 6% Tacsimate (pH 4.5), 18% PEG 3350 |
