7M93

Bovine sigma-2 receptor bound to PB28

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-11-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.254858
Spacegroup name	P 1 21 1
Unit cell lengths	70.597, 55.174, 92.985
Unit cell angles	90.00, 95.05, 90.00

Refinement procedure

Resolution	33.880 - 2.940
R-factor	0.2043
Rwork	0.199
R-free	0.24980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6oht
RMSD bond length	0.003
RMSD bond angle	0.594
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.880	3.047
High resolution limit [Å]	2.940	2.940
Rmeas	0.227
Rpim	0.118	0.585
Number of reflections	15228	760
<I/σ(I)>	5.73	0.93
Completeness [%]	98.7	90.76
Redundancy	4	3.4
CC(1/2)	0.974	0.495

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6	293	PEG 300, 0.1 M MES pH 6, 600 mM NaCl