7M8A

Human DNA Pol eta S113A with rA-ended primer and dATP: in crystallo reaction for 40 s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-22
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 61
Unit cell lengths	98.393, 98.393, 82.025
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.980 - 1.910
R-factor	0.1697
Rwork	0.166
R-free	0.21500
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4ecq
RMSD bond length	0.007
RMSD bond angle	0.931
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.940
High resolution limit [Å]	1.910	5.180	1.910
Rmerge	0.077	0.027	0.587
Total number of observations	94008
Number of reflections	34880	1736	1700
<I/σ(I)>	9.6
Completeness [%]	99.4	95.8	99.1
Redundancy	2.7	2.9	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	295	15% PEG 2000 MME, 100 mM MES