7M84

Human DNA Pol eta S113A with dA-ended primer and dATP: in crystallo reaction for 40 s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-22
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 61
Unit cell lengths	98.104, 98.104, 82.126
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.910 - 1.470
R-factor	0.1744
Rwork	0.172
R-free	0.19940
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4ecq
RMSD bond length	0.007
RMSD bond angle	1.098
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.500
High resolution limit [Å]	1.470	3.990	1.470
Rmerge	0.060	0.021	0.676
Total number of observations	281629
Number of reflections	76255	3847	3780
<I/σ(I)>	12.7
Completeness [%]	99.9	98.3	99.9
Redundancy	3.7	3.7	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	295	15% PEG 2000 MME, 100 mM MES