7M81

Human DNA Pol eta with dA-ended primer and dATP: in crystallo reaction for 100 s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-30
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 61
Unit cell lengths	99.147, 99.147, 81.761
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.160 - 2.050
R-factor	0.1748
Rwork	0.171
R-free	0.22630
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4ecq
RMSD bond length	0.008
RMSD bond angle	1.213
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.090
High resolution limit [Å]	2.050	5.560	2.050
Rmerge	0.090	0.026	0.641
Total number of observations	107497
Number of reflections	28509	1412	1422
<I/σ(I)>	8
Completeness [%]	98.8	94.3	99.2
Redundancy	3.8	3.8	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	295	15% PEG 2000 MME, 100 mM MES