7M7S

Human DNA Pol eta S113A with dT-ended primer and 0.1 mM dAMPNPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 61
Unit cell lengths	98.676, 98.676, 81.832
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.490 - 1.850
R-factor	0.1837
Rwork	0.180
R-free	0.22830
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4ecq
RMSD bond length	0.006
RMSD bond angle	0.889
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	1.880
High resolution limit [Å]	1.850	5.020	1.850
Rmerge	0.082	0.026	0.628
Total number of observations	143896
Number of reflections	38744	2000	1925
<I/σ(I)>	9.3
Completeness [%]	100.0	99.8	100
Redundancy	3.7	3.7	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	295	15% PEG 2000 MME, 100 mM MES