7M7Q

Human DNA Pol eta S113A with dT-ended primer and dAMPNPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 61
Unit cell lengths	98.927, 98.927, 81.807
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.380 - 2.270
R-factor	0.1732
Rwork	0.169
R-free	0.22390
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4ecq
RMSD bond length	0.010
RMSD bond angle	1.323
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	2.310
High resolution limit [Å]	2.270	6.150	2.270
Rmerge	0.154	0.032	0.686
Total number of observations	80413
Number of reflections	20884	1012	1060
<I/σ(I)>	5.9
Completeness [%]	98.3	91.7	99.9
Redundancy	3.9	4	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6	295	15% PEG 2000 MME, 100 mM MES