7M54

Crystallographic structure of a cubic crystal form of STMV grown from bromide

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	173
Detector technology	CCD
Collection date	2002-06-21
Detector	MAR CCD 165 mm
Wavelength(s)	1.0
Spacegroup name	P 2 3
Unit cell lengths	234.650, 234.650, 234.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.910 - 3.800
R-factor	0.2188
Rwork	0.213
R-free	0.28530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4oq8
RMSD bond length	0.005
RMSD bond angle	0.649
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	105.000	3.650
High resolution limit [Å]	3.500	3.500
Rmerge	0.210	0.320
Number of reflections	38017	1200
<I/σ(I)>	7.7	1.1
Completeness [%]	73.0
Redundancy	4.6
CC(1/2)	0.940	0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	279	crystals were grown by sitting drop vapor diffusion in Cryschem plates using 0.6 ml reservoirs. Drops were 6 to 8 ul and composed of equal volumes of a 5 mg/ml virus stock solution buffered at pH 6.5 with 0.1M phosphate, and the reservoir solution. The reservoir solution was 18% w/v NaBr buffered with 0.1 M phosphate at pH 6.0. Crystallization was carried out at 4 degrees C.