7M50

Crystallographic structure of a cubic crystal form of STMV grown from ammonium sulfate

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	173
Detector technology	CCD
Collection date	2002-06-20
Detector	MAR CCD 165 mm
Wavelength(s)	1.0
Spacegroup name	P 2 3
Unit cell lengths	234.050, 234.050, 234.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.010 - 2.310
R-factor	0.2484
Rwork	0.246
R-free	0.27080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4oq8
RMSD bond length	0.002
RMSD bond angle	0.490
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.000	2.350
High resolution limit [Å]	2.300	2.300
Rmerge	0.180	0.320
Number of reflections	159229	854
<I/σ(I)>	12.8	1.3
Completeness [%]	1.0	0.25
Redundancy	10.17	1.6
CC(1/2)	0.910	0.410

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	279	Crystals were grown by sitting drop vapor diffusion in Cryschem plates using 0.6 ml reservoirs. Drops were 6 to 8 ul and were composed of equal volumes of a 5 mg/ml virus stock solution containing 0.1 M phosphate at pH 6.5, and the reservoir solution. The reservoir solution was 18% ammonium sulfate in 0.1 M phosphate at pH 6.0. Crystallization was carried out at 4 degrees C.