7M4H

DNA Polymerase Lambda, dCTP:At Mn2+ Reaction State Ternary Complex, 225 min

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-08-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	55.814, 62.299, 142.057
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.571 - 1.923
R-factor	0.194
Rwork	0.192
R-free	0.22750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3upq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX (v1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.960
High resolution limit [Å]	1.923	5.240	1.930
Rmerge	0.085	0.034	0.938
Rmeas	0.093	0.038	1.051
Rpim	0.038	0.016	0.467
Number of reflections	38316	2098	1866
<I/σ(I)>	8.7
Completeness [%]	100.0	99.8	99.7
Redundancy	5.8	5.6	4.9
CC(1/2)		0.998	0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	80-95mM BICINE pH 8.3, 0.3M Na-K Tartrate, 18-22.5% PolyPure PEG