7M4G

DNA Polymerase Lambda, dCTP:At Mg2+ Product State Ternary Complex, 960 min

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-03
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	55.997, 62.235, 141.114
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.926 - 1.880
R-factor	0.2129
Rwork	0.211
R-free	0.23950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3upq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX (v1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.910
High resolution limit [Å]	1.880	5.100	1.880
Rmerge	0.050	0.033	0.826
Rmeas	0.055	0.037	0.911
Rpim	0.023	0.016	0.380
Total number of observations	239319
Number of reflections	40938	2235	1982
<I/σ(I)>	13.5
Completeness [%]	99.8	99.5	99.3
Redundancy	5.8	5.6	5.5
CC(1/2)		0.997	0.830

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	80-95mM BICINE pH 8.3, 0.3M Na-K Tartrate, 18-22.5% PolyPure PEG