7M4F

DNA Polymerase Lambda, dCTP:At Mg2+ Product State Ternary Complex, 300 min

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-12-07
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	55.869, 62.065, 142.087
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.128 - 1.947
R-factor	0.2091
Rwork	0.207
R-free	0.24090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3upq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX (v1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.980
High resolution limit [Å]	1.947	5.290	1.950
Rmerge	0.063	0.061	0.559
Rmeas	0.069	0.067	0.612
Rpim	0.028	0.027	0.244
Number of reflections	36574	1978	1631
<I/σ(I)>	11.2
Completeness [%]	98.8	96.9	90.7
Redundancy	6.1	5.6	5.8
CC(1/2)		0.995	0.924

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	80-95mM BICINE pH 8.3, 0.3M Na-K Tartrate, 18-22.5% PolyPure PEG