7M4C

DNA Polymerase Lambda, TTP:At Mn2+ Product State Ternary Complex, 960 min

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-22
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	56.021, 62.641, 140.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.874 - 1.950
R-factor	0.1828
Rwork	0.181
R-free	0.21410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3upq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX (v1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.980
High resolution limit [Å]	1.950	5.290	1.950
Rmerge	0.095	0.039	0.967
Rmeas	0.098	0.042
Rpim	0.034	0.015	0.345
Total number of observations	323798
Number of reflections	36795	2010	1785
<I/σ(I)>	7.4
Completeness [%]	100.0	99.7	100
Redundancy	8.8	8.1	8.7
CC(1/2)		0.998	0.829

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	80-95mM BICINE pH 8.3, 0.3M Na-K Tartrate, 18-22.5% PolyPure PEG