7M4B

DNA Polymerase Lambda, TTP:At Mn2+ Product State Ternary Complex, 60 min

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-22
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	55.981, 62.634, 140.334
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.144 - 1.660
R-factor	0.1817
Rwork	0.181
R-free	0.20170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3upq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX (v1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.690
High resolution limit [Å]	1.660	4.500	1.660
Rmerge	0.067	0.062	0.474
Rmeas	0.073	0.068	0.517
Rpim	0.028	0.027	0.203
Total number of observations	416607
Number of reflections	58724	3132	2672
<I/σ(I)>	12.5
Completeness [%]	99.1	97.3	90.9
Redundancy	7.1	6.5	5.9
CC(1/2)		0.991	0.943

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	80-95mM BICINE pH 8.3, 0.3M Na-K Tartrate, 18-22.5% PolyPure PEG