7M49

DNA Polymerase Lambda, TTP:At Mn2+ Reaction State Ternary Complex, 5 min

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-07
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	56.146, 62.771, 140.568
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.108 - 1.600
R-factor	0.1889
Rwork	0.188
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3upq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX (v1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.630
High resolution limit [Å]	1.600	4.340	1.600
Rmerge	0.053	0.040	0.774
Rmeas	0.055	0.042	0.817
Rpim	0.017	0.013	0.254
Total number of observations	687183
Number of reflections	66149	3587	3055
<I/σ(I)>	14
Completeness [%]	99.5	99.5	93.8
Redundancy	10.4	9.7	9.3
CC(1/2)		0.998	0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	80-95mM BICINE pH 8.3, 0.3M Na-K Tartrate, 18-22.5% PolyPure PEG