7M48

DNA Polymerase Lambda, TTP:At Mg2+ Product State Ternary Complex, 960 min

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	56.168, 62.653, 139.912
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.896 - 1.930
R-factor	0.1857
Rwork	0.184
R-free	0.22040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3upq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX (v1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.960
High resolution limit [Å]	1.930	5.240	1.930
Rmerge	0.089	0.038	0.945
Rmeas	0.093	0.041
Rpim	0.034	0.014	0.372
Total number of observations	281178
Number of reflections	37937	2043	1886
<I/σ(I)>	7.8
Completeness [%]	99.8	98	99.9
Redundancy	7.4	7.1	7.2
CC(1/2)		0.999	0.793

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	80-95mM BICINE pH 8.3, 0.3M Na-K Tartrate, 18-22.5% PolyPure PEG