7M46

DNA Polymerase Lambda, TTP:At Mg2+ Product State Ternary Complex, 5 min

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-29
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	56.263, 62.808, 139.452
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.620 - 1.920
R-factor	0.1876
Rwork	0.186
R-free	0.21650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3upq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX (v1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.950
High resolution limit [Å]	1.920	5.210	1.920
Rmerge	0.057	0.037	0.591
Total number of observations	227329
Number of reflections	38559	2083	1877
<I/σ(I)>	15.4
Completeness [%]	99.8	98.4	98.6
Redundancy	5.9	6.3	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	80-95mM BICINE pH 8.3, 0.3M Na-K Tartrate, 18-22.5% PolyPure PEG