7M3V

RNA bulged-G motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.10567
Spacegroup name	P 43
Unit cell lengths	29.657, 29.657, 76.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.660 - 2.190
R-factor	0.1814
Rwork	0.177
R-free	0.21350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q9a
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.660	27.660	2.250
High resolution limit [Å]	2.190	9.820	2.190
Rmerge	0.118	0.079	0.335
Rmeas	0.128	0.087	0.368
Total number of observations	45236
Number of reflections	6691	68	500
<I/σ(I)>	13.64	20.45	7.22
Completeness [%]	99.8	98.6	97.7
Redundancy	6.761	6.235	5.992
CC(1/2)	0.995	0.997	0.932

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	MOPS, ammonium sulfate, magnesium chloride