7M3T

Crystallographic structure of a cubic crystal of STMV (80.7 degree rotation about 111) grown from chloride

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	173
Detector technology	CCD
Collection date	2002-07-15
Detector	MAR CCD 165 mm
Wavelength(s)	1.0
Spacegroup name	P 2 3
Unit cell lengths	234.050, 234.050, 234.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.810 - 3.200
R-factor	0.2184
Rwork	0.215
R-free	0.25360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4oq8
RMSD bond length	0.003
RMSD bond angle	0.553
Data reduction software	d*TREK
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.000	3.300
High resolution limit [Å]	3.200	3.200
Rmerge	0.230	0.350
Number of reflections	69957	1238
<I/σ(I)>	9.9	2.2
Completeness [%]	99.0
Redundancy	9.55
CC(1/2)	0.997	0.430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	279	Crystals were grown by sitting drop vapor diffusion in Cryschem plates using 0.6 ml reservoirs. The drops were equal volumes of 5 mg/ml STMV in 0.1 M phosphate at pH 6.5 and the reservoir solution, which was 8% w/v NaCl in 0.1 M phosphate at pH 6.0. Crystallization was carried out at 4 degrees C.