7M2T

Crystallographic Structure of the Monoclinic Form of Satellite Tobacco Mosaic Virus

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1992-06-15
Detector	SDMS
Wavelength(s)	1.54
Spacegroup name	I 1 2 1
Unit cell lengths	175.760, 169.860, 244.560
Unit cell angles	90.00, 92.69, 90.00

Refinement procedure

Resolution	49.950 - 2.710
R-factor	0.1316
Rwork	0.129
R-free	0.17670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4oq8
RMSD bond length	0.003
RMSD bond angle	0.529
Data reduction software	SDMS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.950	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.069	0.134
Rpim	0.036
Number of reflections	179156	11858
<I/σ(I)>	9.27	5.7
Completeness [%]	91.8	58.8
Redundancy	8.2
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	279	Crystals grown by vapor diffusion as sitting drops. Drops were equal volumes of a 6 mg/ml STMV stock solution buffered at pH 6.5 with 0.1 M phosphate. The reservoirs were 16% saturated ammonium sulfate in 0.1 M phosphate at pH 6.5. Drops were 6 to 8 ul with 0.60 ml reservoirs. Crystals were grown at 4 degrees C over a weeks time.