7M1U

Crystal structure of an archaeal CNNM, MtCorB, R235L mutant with C-terminal deletion

Replaces:  7LJ8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.52154
Spacegroup name	C 1 2 1
Unit cell lengths	139.168, 124.024, 86.261
Unit cell angles	90.00, 92.81, 90.00

Refinement procedure

Resolution	46.270 - 3.800
R-factor	0.2448
Rwork	0.243
R-free	0.28500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7LJ6 7LJ7
RMSD bond length	0.002
RMSD bond angle	0.438
Data reduction software	HKL-2000 (v7.20)
Data scaling software	HKL-2000 (v7.20)
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.940
High resolution limit [Å]	3.800	3.800
Number of reflections	15203	152
<I/σ(I)>	16.3	0.8
Completeness [%]	91.7	78.7
Redundancy	6	4.5
CC(1/2)	0.965	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1 M sodium citrate, pH 5.5; 50 mM Li2SO4; 11% PEG4000; 10 mM MgSO4