7M1T

Crystal structure of an archaeal CNNM, MtCorB, with C-terminal deletion in complex with Mg2+-ATP

Replaces:  7LJ7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97996
Spacegroup name	P 21 21 21
Unit cell lengths	61.050, 118.676, 177.305
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.310 - 3.260
R-factor	0.2279
Rwork	0.226
R-free	0.26670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7LJ6
RMSD bond length	0.002
RMSD bond angle	0.498
Data reduction software	HKL-2000 (v720)
Data scaling software	HKL-2000 (v720)
Phasing software	PHENIX (1.17_3660)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.250	3.250
Number of reflections	15717	75
<I/σ(I)>	22	1
Completeness [%]	99.2	96.9
Redundancy	11.9	9.1
CC(1/2)	0.993	0.430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1 M sodium citrate, pH 5.5; 0.1 M Li2SO4; 0.1 M NaCl; 20 mM MgCl2, 34% PEG400; 10 mM Na2HPO4; 5 mM ATP