7M1L

Crystal structure of Pseudomonas aeruginosa ClpP2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	124.372, 147.439, 99.338
Unit cell angles	90.00, 121.73, 90.00

Refinement procedure

Resolution	19.970 - 2.000
R-factor	0.198
Rwork	0.197
R-free	0.21750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yg6
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.073	0.042	0.931
Rmeas	0.085	0.050	1.092
Rpim	0.044	0.026	0.566
Total number of observations	383187
Number of reflections	102184	4849	5097
<I/σ(I)>	7.3
Completeness [%]	99.6	92.9	100
Redundancy	3.7	3.6	3.7
CC(1/2)		0.996	0.624

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	291	1.8 M sodium phosphate monobasic monohydrate, potassium phosphate dibasic, pH 6.9