Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.650, 48.790, 112.660 |
| Unit cell angles | 90.00, 103.60, 90.00 |
Refinement procedure
| Resolution | 40.487 - 1.500 |
| Rwork | 0.201 |
| R-free | 0.23680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7m1a |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.920 |
| Data reduction software | autoPROC |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.500 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.048 | 0.441 |
| Rpim | 0.021 | 0.193 |
| Number of reflections | 63258 | 9179 |
| <I/σ(I)> | 18.3 | 3.6 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.1 | 6.1 |
| CC(1/2) | 0.990 | 0.939 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 24% w/v PEG4000, 8.0% v/v ethylene glycol, 0.4 M magnesium chloride, 100 mM Tris:HCl, pH 8.5 |
